QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒12381-上海力敏實(shí)業(yè)有限公司

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QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

更新時(shí)間： 2023-07-26
產(chǎn)品型號(hào)： 12381
簡(jiǎn)單描述
QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒
EndoFree Plasmid Kits基于陰離子交換技術(shù)，可快速制備不含內(nèi)毒素的質(zhì)粒DNA。QIAfilter Cartridges過(guò)濾快速澄清裂解液。制備的DNA純度超過(guò)兩次CsCl梯度離心法所得DNA的純度，適合超轉(zhuǎn)染級(jí)純的應(yīng)用。EndoFree Plasmid Buffer Set可用于制備10次m

詳細(xì)介紹

QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

純化得到至多10 mg的無(wú)內(nèi)毒素高轉(zhuǎn)染級(jí)純質(zhì)粒或柯斯質(zhì)粒DNA

純化得到的DNA內(nèi)毒素水平低于0.1 EU/µg
純化流程快，同時(shí)去除內(nèi)毒素
獲得高產(chǎn)量、高拷貝質(zhì)粒DNA
使用LyseBlue，獲得的裂解和高產(chǎn)量的DNA

QIAGEN EndoFree Plasmid Mega Kit無(wú)內(nèi)毒素質(zhì)粒提取試劑盒

質(zhì)粒純化方法與轉(zhuǎn)染效率。

使用兩種方法獨(dú)立制備pRSVcat，分別使用脂質(zhì)體介導(dǎo)的方法轉(zhuǎn)染到COS-7、HeLa和LMH細(xì)胞中，及使用磷酸鈣轉(zhuǎn)染至Huh7細(xì)胞中。平均轉(zhuǎn)染效率以相對(duì)于QIAGEN Plasmid Kit（100％）制備的DNA的轉(zhuǎn)染效率表示。

質(zhì)粒純化方法與轉(zhuǎn)染效率。

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QIAGEN Plasmid Plus Midi Kit (25)	3600	12943	25
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QIAGEN Plasmid Plus Maxi Sample Kit (5)	1490	12961
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QIAGEN Blood&CellCulture DNA MaxiKit(10)	5070	13362	10
QIAGEN Plasmid Plus 96 Miniprep Kit (4)	35860	16181	384
QIArack	1250	19015
Plasmid Buffer Set	1410	19046
EndoFree Plasmid Buffer Set	7430	19048
Buffer P1 (500 ml)	811	19051	500
Buffer P2 (500ml)	811	19052	500
Buffer P3 (500ml)	811	19053	500
Buffer QBT (1000ml)	1410	19054	1000
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Buffer PB (500ml)	1060	19066	500
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Buffer AVL (155 ml)	1900	19073
Buffer AL	1220	19075	1
Buffer ATL (200ml)	1220	19076	200
Buffer AE (240ml)	1220	19077	1
Buffer AW1 (concentrate, 242 ml)	1220	19081	1
Buffer ASL (4 x 140 ml)	1370	19082
Buffer PM (500ml)	837	19083	500
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QIAvac Connecting System	13950	19419
QIAvac 96	10200	19504
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QIAEX II Gel Extraction Kit (150)	2340	20021	150
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